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segmentSeq

Methods for identifying small RNA loci from high-throughput sequencing data


Bioconductor version: Release (3.18)

High-throughput sequencing technologies allow the production of large volumes of short sequences, which can be aligned to the genome to create a set of matches to the genome. By looking for regions of the genome which to which there are high densities of matches, we can infer a segmentation of the genome into regions of biological significance. The methods in this package allow the simultaneous segmentation of data from multiple samples, taking into account replicate data, in order to create a consensus segmentation. This has obvious applications in a number of classes of sequencing experiments, particularly in the discovery of small RNA loci and novel mRNA transcriptome discovery.

Author: Thomas J. Hardcastle

Maintainer: Thomas J. Hardcastle <tjh48 at cam.ac.uk>

Citation (from within R, enter citation("segmentSeq")):

Installation

To install this package, start R (version "4.3") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("segmentSeq")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("segmentSeq")
segmentSeq: small RNA locus detection PDF R Script
segmentsSeq: Methylation locus identification PDF R Script
Reference Manual PDF

Details

biocViews Alignment, DataImport, DifferentialExpression, MultipleComparison, QualityControl, Sequencing, Software
Version 2.36.0
In Bioconductor since BioC 2.6 (R-2.11) (14 years)
License GPL-3
Depends R (>= 3.5.0), methods, baySeq(>= 2.9.0), S4Vectors, parallel, GenomicRanges, ShortRead, stats
Imports Rsamtools, IRanges, GenomeInfoDb, graphics, grDevices, utils, abind
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package segmentSeq_2.36.0.tar.gz
Windows Binary segmentSeq_2.36.0.zip
macOS Binary (x86_64) segmentSeq_2.36.0.tgz
macOS Binary (arm64) segmentSeq_2.36.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/segmentSeq
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/segmentSeq
Bioc Package Browser https://code.bioconductor.org/browse/segmentSeq/
Package Short Url https://bioconductor.org/packages/segmentSeq/
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