1 Introduction

2 Background information

3 Illustrating dataset

4 Specifying the pipeline

5 Running the pipeline

6 Visualizing the results

7 Comparing pipelines

8 Example with two different QC methods

9 Visualizing scale transformations

10 Defining technical run parameters

Session information

## R version 4.4.0 RC (2024-04-16 r86468)
## Platform: x86_64-pc-linux-gnu
## Running under: Ubuntu 22.04.4 LTS
## 
## Matrix products: default
## BLAS:   /home/biocbuild/bbs-3.20-bioc/R/lib/libRblas.so 
## LAPACK: /usr/lib/x86_64-linux-gnu/lapack/liblapack.so.3.10.0
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_GB              LC_COLLATE=C              
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## time zone: America/New_York
## tzcode source: system (glibc)
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
## [1] patchwork_1.2.0       CytoPipelineGUI_1.3.0 CytoPipeline_1.5.0   
## [4] BiocStyle_2.33.0     
## 
## loaded via a namespace (and not attached):
##   [1] DBI_1.2.2             gridExtra_2.3         rlang_1.1.3          
##   [4] magrittr_2.0.3        clue_0.3-65           GetoptLong_1.0.5     
##   [7] matrixStats_1.3.0     compiler_4.4.0        RSQLite_2.3.6        
##  [10] png_0.1-8             vctrs_0.6.5           reshape2_1.4.4       
##  [13] stringr_1.5.1         pkgconfig_2.0.3       shape_1.4.6.1        
##  [16] crayon_1.5.2          fastmap_1.1.1         magick_2.8.3         
##  [19] dbplyr_2.5.0          labeling_0.4.3        utf8_1.2.4           
##  [22] promises_1.3.0        ncdfFlow_2.51.0       rmarkdown_2.26       
##  [25] graph_1.83.0          tinytex_0.50          purrr_1.0.2          
##  [28] bit_4.0.5             xfun_0.43             zlibbioc_1.51.0      
##  [31] cachem_1.0.8          jsonlite_1.8.8        flowWorkspace_4.17.0 
##  [34] blob_1.2.4            highr_0.10            later_1.3.2          
##  [37] parallel_4.4.0        cluster_2.1.6         R6_2.5.1             
##  [40] bslib_0.7.0           stringi_1.8.3         RColorBrewer_1.1-3   
##  [43] jquerylib_0.1.4       Rcpp_1.0.12           bookdown_0.39        
##  [46] iterators_1.0.14      knitr_1.46            zoo_1.8-12           
##  [49] IRanges_2.39.0        flowCore_2.17.0       httpuv_1.6.15        
##  [52] tidyselect_1.2.1      yaml_2.3.8            doParallel_1.0.17    
##  [55] codetools_0.2-20      curl_5.2.1            lattice_0.22-6       
##  [58] tibble_3.2.1          plyr_1.8.9            Biobase_2.65.0       
##  [61] shiny_1.8.1.1         withr_3.0.0           evaluate_0.23        
##  [64] BiocFileCache_2.13.0  circlize_0.4.16       pillar_1.9.0         
##  [67] BiocManager_1.30.22   filelock_1.0.3        foreach_1.5.2        
##  [70] flowAI_1.35.0         stats4_4.4.0          generics_0.1.3       
##  [73] diagram_1.6.5         S4Vectors_0.43.0      ggplot2_3.5.1        
##  [76] munsell_0.5.1         ggcyto_1.33.0         scales_1.3.0         
##  [79] xtable_1.8-4          PeacoQC_1.15.0        glue_1.7.0           
##  [82] changepoint_2.2.4     tools_4.4.0           hexbin_1.28.3        
##  [85] data.table_1.15.4     XML_3.99-0.16.1       grid_4.4.0           
##  [88] RProtoBufLib_2.17.0   colorspace_2.1-0      cli_3.6.2            
##  [91] fansi_1.0.6           cytolib_2.17.0        ComplexHeatmap_2.21.0
##  [94] dplyr_1.1.4           Rgraphviz_2.49.0      gtable_0.3.5         
##  [97] sass_0.4.9            digest_0.6.35         BiocGenerics_0.51.0  
## [100] farver_2.1.1          rjson_0.2.21          memoise_2.0.1        
## [103] htmltools_0.5.8.1     lifecycle_1.0.4       httr_1.4.7           
## [106] GlobalOptions_0.1.2   mime_0.12             bit64_4.0.5