1 Workflow version information

R version: R version 4.0.0 RC (2020-04-19 r78255)

Bioconductor version: 3.12

Package: 1.13.0

2 Introduction

Chromatin immunoprecipitation with sequencing (ChIP-seq) is a widely used technique for identifying the genomic binding sites of a target protein. Conventional analyses of ChIP-seq data aim to detect absolute binding (i.e., the presence or absence of a binding site) based on peaks in the read coverage. An alternative analysis strategy is to detect of changes in the binding profile between conditions (Ross-Innes et al. 2012; Pal et al. 2013). These differential binding (DB) analyses involve counting reads into genomic intervals and testing those counts for significant differences between conditions. This defines a set of putative DB regions for further examination. DB analyses are statistically easier to perform than their conventional counterparts, as the effect of genomic biases is largely mitigated when counts for different libraries are compared at the same genomic region. DB regions may also be more relevant as the change in binding can be associated with the biological difference between conditions.

The key step in the DB analysis is the manner in which reads are counted. The most obvious strategy is to count reads into pre-defined regions of interest, like promoters or gene bodies (Pal et al. 2013). This is simple but will not capture changes outside of those regions. In contrast, de novo analyses do not depend on pre-specified regions, instead using empirically defined peaks or sliding windows for read counting. Peak-based methods are implemented in the DiffBind and DBChIP software packages (Ross-Innes et al. 2012; Liang and Keles 2012), which count reads into peak intervals that have been identified with software like MACS (Zhang et al. 2008). This requires some care to maintain statistical rigour as peaks are called with the same data used to test for DB. Alternatively, window-based approaches count reads into sliding windows across the genome. This is a more direct strategy that avoids problems with data re-use and can provide increased DB detection power (Lun and Smyth 2014). However, its correct implementation is not straightforward due to the subtleties with interpretation of the false discovery rate (FDR).

3 Differential binding with sliding windows

Here, we describe computational workflows for performing a DB analysis with sliding windows. It is primarily based on the csaw software package but also uses a number of other packages from the open-source Bioconductor project (Huber et al. 2015). The aim is to facilitate the practical implementation of window-based DB analyses by providing detailed code and expected output. We demonstrate on data from real studies examining changes in transcription factor binding (Kasper et al. 2014) and histone mark enrichment (Revilla-I-Domingo et al. 2012).

The workflows described here apply to any ChIP-seq experiment with multiple experimental conditions and with multiple biological samples within one or more of the conditions. They detect and summarize DB regions between conditions in a de novo manner, i.e., without making any prior assumptions about the location or width of bound regions. Detected regions are then annotated according to their proximity to annotated genes. In addition, the code can be easily adapted to accommodate batch effects, covariates and multiple experimental factors.

Note: to cite this article, please refer to https://f1000research.com/articles/4-1080/v2 for instructions.

4 Obtaining BAM files

All of the workflows described here start from sorted and indexed BAM files in the chipseqDBData package. For application to user-specified data, the raw read sequences have to be aligned to the appropriate reference genome beforehand. Most aligners can be used for this purpose, but we have used Rsubread (Liao, Smyth, and Shi 2013) due to the convenience of its R interface. It is also recommended to mark duplicate reads using tools like Picard prior to starting the workflow.

5 Author information

5.1 Author contributions

A.T.T.L. developed and tested the workflow on the H3K9ac and CBP data sets. G.K.S. provided direction on the design of the workflow. Both A.T.T.L. and G.K.S. wrote the article.

5.2 Competing interests

No competing interests were disclosed.

5.3 Grant information

National Health and Medical Research Council (Program Grant 1054618 to G.K.S., Fellowship to G.K.S.); Victorian State Government Operational Infrastructure Support; Australian Government NHMRC IRIIS.

5.4 Acknowledgements

The authors would like to thank Prof. Stephen Nutt for his valuable insights on B-cell biology.

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